INTRACELLULAR CRYSTALLINE ERGOSTEROL IN NEUROSPORA
نویسندگان
چکیده
منابع مشابه
Intracellular Crystalline Ergosterol in Neurospora
In the fungus Neurospora crassa, hexagonal crystalline inclusions have been observed with both the light and electron microscopes. These crystals have been enriched by differential centrifugation and found to be identical with ergosterol by the criteria of ultraviolet spectral analysis and cytochemical analysis. Observations have been made on the distribution and fine structure of the crystalli...
متن کاملIsotopic acetate and isovalerate in the synthesis of ergosterol by Neurospora.
The utilization of deuteroacetate for the synthesis of ergosterol in an acetateless Neurospora mutant has been described in an earlier paper (1). In this work it was demonstrated that at least 25 per cent of the hydrogen atoms of ergosterol is derived directly from acetate. Since the use of deuterium as a tracer necessarily involves errors of unknown magnitude due to the lability of deuterium a...
متن کاملA PARA CRYSTALLINE INCLUSION IN NEUROSPORA CRASSA Induction by Ethidium and Acridine, Isolation, and Characterization
A paracrystal indistinguishable from the one which occurs in the mitochondrial mutant abnormal-1 can be induced in wild-type Neurospora crassa after growth in either ethidium or euflavine . This paracrystal has been isolated and partially characterized . It appears to be composed of a single polypeptide (mol wt 68,000) which can be reversibly crystallized and dissociated by changes in the pH an...
متن کاملIdentification of Ergosterol in Mushrooms
Mushroom has gained popularity as prized table delicacy with high nutritive and medicinal value. Ergosterol is a biological precursor (a provitamin) to vitamin D2. The contents of vitamin D2 and sterols in some wild and cultivated mushrooms were determined. Vitamin D2 was determined using an HPLC method, including saponification and semipreparative normalphase HPLC purification Lv et al. [1]. C...
متن کاملChanges in Intracellular pH Are Not Correlated with the Circadian Rhythm of Neurospora.
Intracellular pH (pH(i)) was measured during the circadian cycle of Neurospora. Internal pH of Neurospora cultures in liquid medium was assayed by the 5,5-dimethyl-2,4-oxazolidinedione method and gave values for pH(i) which were similar to those previously obtained by other workers using pH-microelectrodes with agar-grown cultures. Cytoplasmic pH changed in liquid medium cultures, but these cha...
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ژورنال
عنوان ژورنال: The Journal of Biophysical and Biochemical Cytology
سال: 1961
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.11.1.171